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Image Search Results
Journal: Journal of Leukocyte Biology
Article Title: The generation of macrophages with anti-inflammatory activity in the absence of STAT6 signaling
doi: 10.1189/jlb.2A1114-560R
Figure Lengend Snippet: (A) Chemokine and cytokine secretion by BMDMs was measured by a proteome profiler membrane antibody array. Supernatants from nonstimulated macrophages were compared with macrophages treated with 10 ng/ml LPS or a combination of LPS and 100 µl Ova-IC (L+I), 200 nM PGE2 (L+P), 200 μM Ado (L+A), or 20 ng/ml IL-4 for 12 h. The proteins that are of interest to this study are indicated in circles, and the letters and numbers are provided to identify the position of the analyte in the membrane. The profiling was done on pooled supernatants collected from 3 separate experiments from 3 different mice. (B). Mean fold differences in intensity of the duplicate samples for relevant analytes are compared with supernatants from nonstimulated. The alphanumeric values within parentheses indicate their position in the membrane array. BCA-1, B Cell-attracting chemokine-1; TCA-3, T cell activation-3; KC, keratinocyte-derived chemokine.
Article Snippet:
Techniques: Membrane, Ab Array, Activation Assay, Derivative Assay