Journal: Clinical and Translational Science

Article Title: Immunostimulatory Effects of Mesenchymal Stem Cell‐Derived Neurons: Implications for Stem Cell Therapy in Allogeneic Transplantations

doi: 10.1111/j.1752-8062.2008.00018.x

Figure Lengend Snippet: Baseline cytokine production in mscs and PBmcs . Cell‐free media from uninduced MSCs and unstimulated PBMCs were analyzed for cytokine production using protein arrays. Each experiment was performed four times, each with cells from a different donor. The densitometric scans were normalized to 10, and the normalized alues are presented as the mean ± SD.

Article Snippet: Microarray for cytokines Cell‐free media from MLR reactions with uninduced and induced (8‐ and 12‐day) MSCs were studied for cytokine expressions with human cytokine protein array III (Ray Biotech, Norcross, GA, USA), as described.

Techniques:

Journal: Clinical and Translational Science

Article Title: Immunostimulatory Effects of Mesenchymal Stem Cell‐Derived Neurons: Implications for Stem Cell Therapy in Allogeneic Transplantations

doi: 10.1111/j.1752-8062.2008.00018.x

Figure Lengend Snippet: Cytokine produce in MLRs. Cell‐free media from MLR with uninduced (day 0) and induced MSCs (day 8 and day 12) were analyzed on cytokine arrays. Parallel studies with media from PBMC or MSCs cultured alone are shown in Table 1. Each experiment was performed four times, each with cells from a different donor. The densitometric scans were normalized to 10, and the normalized values are presented as the mean ± SD.

Article Snippet: Microarray for cytokines Cell‐free media from MLR reactions with uninduced and induced (8‐ and 12‐day) MSCs were studied for cytokine expressions with human cytokine protein array III (Ray Biotech, Norcross, GA, USA), as described.

Techniques: Cell Culture

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of Agarwood-NE on the CSE-induced transcription of the pro-inflammatory cytokine IL-8. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The mRNA levels of IL-8 were determined via RT-qPCR. Values are expressed as mean ± SEM ( n = 4, *: p < 0.05; ****: p < 0.0001).

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Incubation, Quantitative RT-PCR

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of Agarwood-NE on the CSE-induced production of pro-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-1α ( A ), IL-1β ( B ), IL-1Ra ( C ), and GDF-15 ( D ) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Protein Array, Incubation

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of agarwood-NE on the CSE-inhibited production of anti-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-10 ( A ), IL-18Bpa ( B ), GH ( C ), VDBP ( D ), relaxin-2 ( E ), IFN-γ ( F ), PDGF-BB ( G ), and TFF3 ( H ) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Protein Array, Incubation